Introduction of Research

Biomacromolecules, such as proteins and nucleic acids, exhibit fluctuations across various timescales in living systems, giving rise to intricate biological phenomena. Understanding these functions requires direct observation of biomolecular structures, dynamics, and interactions within living cells. In this context, structural biology research in cellular environments has gained significant attention, particularly in-cell/in vivo NMR techniques. These methods enable the analysis of protein structures, stability, activity, and post-translational modifications within living cells. However, conventional approaches face difficulties such as molecular weight limitations and low sensitivity, restricting their applicability.

Our research group is developing novel in-cell /in vivo NMR techniques using advanced nuclear spin detection methods, including specific stable isotope labeling strategies. By leveraging these new in-cell NMR approaches, we aim to establish “High-resolution spin cell biology” to uncover the true nature of biomolecules within living cells.