ExCELLS promotes collaborative research with researchers from other institutes and universities. Various researchers can use ExCELLS equipment to perform their own research through joint research projects along with ExCELLS researchers.
ExCELLS welcomes an application of from researchers who want to use ExCELLS’s equipment.
If you have any questions, please feel free to the contact desk (collabo_at_excells.orion.ac.jp).
*Please replace the “_at_” with @
The following is an introduction for equipment available in ExCELLS joint research.
Combined system of high-speed atomic force microscopy and fluorescence microscopy
The combined high-speed atomic force microscopy (HS-AFM) and fluorescence microscopy can visualize the dynamic phenomena of various biological samples from proteins to living cells in real time. This equipment can visualize biomolecular behaviors simultaneously with HS-AFM and fluorescence microscopy.
Q-TOF mass spectrometer for native MS
This equipment is a Q-TOF mass spectrometer. The combination of Electrospray ionization and gentle gradual desolvation makes it possible to perform native Mass Spectroscopy (nMS). nMS is a powerful tool to determine the mass of the entire complex, even for biomolecular complexes formed by non-covalent bonds.
High-speed live imaging system
Spinning disk confocal microscopy equipped with cell culture device. Three lasers (488, 560, 640 nm) are equipped, and long-term live imaging (~ 1week) can be performed.
Total internal reflection fluorescence (TIRF) microscope
Total internal reflection fluorescence (TIRF) lasers (405, 488, 561 nm) are equipped with electric inverted microscope using EM-CCD (Andor) detector. Single molecule measurement and HILO imaging can be performed using 100X TIRF objective lens.
Super-resolution microscopy systems
Multifunctional super-resolution confocal fluorescence microscope
This multifunctional confocal microscope enables super-resolution, fluorescence lifetime measurement, and fluorescence correlation microscopy.
- Super-resolution microscopy
– STED (stimulated emission depletion) can resolve below 50 nm.
– An easy-to-use deconvolution program “Lightning” archive ~120 nm resolution.
- Fluorescence lifetime imaging microscopy (FLIM)
FALCON (FAst Lifetime CONtrast) enables much faster fluorescent lifetime imaging compared to conventional systems, that is applicable for detection of microenvironmental changes using molecular biosensors, and for separation of multiple fluorophores by their lifetime difference.
- Fluorescence Correlation Spectroscopy (FCS)
FCS is a correlation analysis of fluctuation of the fluorescence intensity that provides quantitative parameters of the physics such as concentration, diffusion coefficient, etc. FCS combined with STED and FALCON enables finer quantification.
Two-photon STED microscope
It enables super-resolution microscopic observation of two-photon excited fluorescence and/or fluorescence lifetime imaging.
Biomolecular interaction analysis system
This system detects intermolecular interaction by surface plasmon resonance, enabling comprehensive, high-throughput analysis to obtain quantitative kinetic and affinity data.
4-Dimensional Tissue Imaging System
This equipment enables us to reconstruct tissue dynamics at the 4-dimensional level by measuring structural and functional changes of tissue and organ of your interest during the developmental or pathological processes using small animals (e.g., mouse and rat).
Cell sorting and measurement system
MA900 Multi-Application Cell Sorter allows detection of up to 12 fluorescence at the single-cell level using fluorescent proteins and/or antibodies labeled with fluorescent dyes, and the gated target cells can be sorted for further analysis.
The cell sorter can automatically adjust various sorting settings (laser beam, optical axis adjustment, electrical timing adjustment for sorting, side stream adjustment, collection tube position adjustment). Four excitation lasers – 405 nm, 488 nm, 561 nm, 638 nm – are equipped, and 96 wells and 384 well plates are available for cell sorting.
Single-cell multi-omics analysis system
A single cell (or cell nucleus) is mixed with molecularly barcoded gel beads in a microdroplet to form tens of thousands of droplets in the device (Chromium system, 10x Genomics inc.). By applying the
appropriate treatment within each microdroplet, multi-omics information such as gene expression, chromatin accessibility, and cell surface protein information within a single cell can be obtained by
combining with NGS (Next Generation Sequencing). Up to 80,000 single cell information can be acquired in a single experiment.
Dynamic Light Scattering instrument
DynaPro Nanostar (Wyatt technology) is a device to measure the size distribution of biomolecules such as proteins and nano particles using Dynamic Light Scattering (DLS). We can also measure molecular weight of biomolecules and viscosity of solutions. For measurements, only several µL of sample is required and a wide range of temperature (−15℃ ~ 150℃) is available.
Laser-Based quartz glass micropipette puller
P-2000 micropipette puller (Sutter) is equipped with a CO2 laser-based heat source and can manufacture quartz glass pipettes. Quartz glass has extremely high physical strength and can achieve a tip diameter of 0.01 micron or less, which is not possible with ordinary glass. In addition, the CO2 laser is not easily affected by the external environment such as temperature and humidity, and has high reproducibility.