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Introduction of Research
Using zebrafish, we are studying the morphology and functional properties of spinal neurons that express a particular transcription factor. Central to our approach is to visualize transcription factor positive cells by making transgenic zebrafish that express fluorescent proteins in these cells. Such transgenic fish allow us to trace development of specific types of neurons, and allow us to perform targeted electrophysiological recordings. We also use optogenetic tools such as ChR2 and halo-rhodopsin to manipulate activity of specific types of neurons.