生命分子動態計測グループ

スタッフ

客員教授:内橋 貴之 (UCHIHASHI, Takayuki)
CV: 履歴はこちら (外部サイトが開きます)
学位:博士(工学)
 
特任助教:渡辺 大輝 (WATANABE, Hiroki)
CV: 履歴はこちら
学位:博士(理学)

 

 

 

研究内容

Dynamic events on a molecular level are essential for a living system because biological molecules fulfil a wide variety of unique functions through conformational change and molecular interactions triggered by binding of ligand/substrate and changes in the external environment. We aim to elucidate function mechanisms of proteins from the aspect of single-molecule dynamics based on direct visualization using high-speed atomic force microscopy (HS-AFM), which enables real-time imaging of individual molecules in action. Further, we carry out functional extensions of the HS-AFM towards imaging dynamics of morphology and mechanical property of a living-cell.

 

連携研究メンバー

岡嶋 孝治(北海道大学大学院情報科学科 教授)
阿部 真之(大阪大学大学院基礎工学研究科 附属極限科学センター 教授)
矢木 宏和(名古屋市立大学大学院薬学研究科 講師)

 

Reference

1. Mori T, et al, Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time. Nat Commun, 2018, 9, 3245
 
2. Uchihashi T, et al, Dynamic structural states of ClpB involved in its disaggregation function”, Nat Commun. 2018, 9, 2147
 
3. Kozai T, et al, Two-step process for disassembly mechanism of proteasome α7 homo-tetradecamer by α6 revealed by high-speed atomic force microscopy, Sci Rep. 2017, 7, 15373
 
4. Shibata M, et al, Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy”, Nat Commun. 2017, 8, 1430

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