MEMBERS

Staff

Visiting Professor: Takayuki UCHIHASHI, Ph.D.
(Affiliation: Nagoya University)
E-mail: uchihast[at]d.phys.nagoya-u.ac.jp
LINK: CV (External site opens in a new window)
LINK: Web Site of Uchihashi Group at Nagoya Univ.(External site opens in a new window)
 
Specially Appointed Assistant Professor: Hiroki WATANABE, Ph.D.
E-mail: hwatanabe[at]ims.ac.jp
LINK: CV

 

 

 

Research

Dynamic events on a molecular level are essential for a living system because biological molecules fulfil a wide variety of unique functions through conformational change and molecular interactions triggered by binding of ligand/substrate and changes in the external environment. We aim to elucidate function mechanisms of proteins from the aspect of single-molecule dynamics based on direct visualization using high-speed atomic force microscopy (HS-AFM), which enables real-time imaging of individual molecules in action. Further, we carry out functional extensions of the HS-AFM towards imaging dynamics of morphology and mechanical property of a living-cell.

 

Collaborate member

Dr. Takaharu Okajima (Professor, Graduate School of Information Science and Technology, Hokkaido University)
 
Dr. Masayuki Abe (Professor, Center for Science and Technology under Extreme Conditions, Osaka University)
 
Dr. Hirokizu Yagi (Lecturer, Graduate School of Pharmaceutical Sciences Department of Structural Biology and Biomolecular Engineering, Nagoya City University)

 

Reference

1. Mori T, et al, Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time. Nat Commun, 2018, 9, 3245
 
2. Uchihashi T, et al, Dynamic structural states of ClpB involved in its disaggregation function”, Nat Commun. 2018, 9, 2147
 
3. Kozai T, et al, Two-step process for disassembly mechanism of proteasome α7 homo-tetradecamer by α6 revealed by high-speed atomic force microscopy, Sci Rep. 2017, 7, 15373
 
4. Shibata M, et al, Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy”, Nat Commun. 2017, 8, 1430

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