MEMBERS

Staff

Specially Appointed Associate Professor: Shigenori NONAKA, Ph.D.
E-mail: snonaka[at]nibb.ac.jp
 

 
LINK: Nonaka Group Website
 

Research

Our main interest is the mechanism how the initial left-right asymmetry is determined in mammalian development. A small patch in a gastrulating mouse embryo called ‘the node’ generates leftward fluid flow by vortical motion of primary cilia on its ventral surface, and the flow direction is critically important to subsequent asymmetric gene expression and final organ arrangement. We are currently focused on the mechanism how the flow is converted to the asymmetric gene expression, i.e. what kind of information is conveyed by the flow, chemical or mechanical or something else, by combining techniques including whole embryo culture, light-sheet microscopy, etc.

 

Reference

・Ichikawa, T., Nakazato, K., Keller, P.J., Kajiura-Kobayashi, H., Stelzer, E.H., Mochizuki, A., and Nonaka, S. Live imaging and quantitative analysis of gastrulation in mouse embryos using light-sheet microscopy and 3D tracking tools. Nat Protoc 9, 575-585. (2014)

・Takao, D., Nemoto, T., Abe, T., Kiyonari, H., Kajiura-Kobayashi, H., Shiratori, H., and Nonaka, S. Asymmetric distribution of dynamic calcium signals in the node of mouse embryo during left-right axis formation. Developmental Biology 376, 23-30. (2013)

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