MEMBERS

Staff

Specially Appointed Associate Professor: Yusuke MIYANARI, Ph.D.
E-mail: miyanari[at]nibb.ac.jp
 

 
LINK: Miyanari Group Web Site
 

Research

We have just open a new lab in early 2014. A fundamental question in biology for us is to understand the mechanisms underlying cell-fate decision. Genomic reprogramming after mammalian fertilization reverts terminally differentiated gametes into toti- or pluripotent state to start a new developmental program. The cell lineage allocation is accompanied by drastic changes in the pattern of gene expression, epigenetic configurations, and nuclear organization. We are interested in a “microscopic” view of the nuclear architecture, which is subnuclear distribution of chromatin and studying functional roles of nuclear dynamics in cell lineage-allocation by deciphering the molecular mechanisms underlying remodeling of nuclear organization and their effects on developmental gene expression, using mouse embryos and ES cells as model systems.
 

Reference

1. Miyanari Y,
A New Approach to Dissect Nuclear Organization:
TALE-Mediated Genome Visualization (TGV). Methods
Mol Biol. 2016;1338:89-97.
 
2. Miyanari Y,
TAL effector-mediated Genome Visualization (TGV)
Methods, 2014, Sep;69(2):198-204.
 
3. Miyanari Y#, Birling CZ. And Torres-Padilla ME*,
Live visualization of chromatin dynamics using
fluorescent TALEs, Nature Structural & Molecular
Biology. 2013 Nov;20(11):1321-4.
#Corresponding authors. Highlighted by Nature Methods.
 
4. Li Y#, Miyanari Y#, Shirane K, Nitta H, Kubota T, Ohashi H,
Okamoto A, Sasaki H. Sequence-specific microscopic
visualization of DNA methylation status at satellite
repeats
in individual cell nuclei and chromosomes,Nucleic Acids
Res. 2013 Oct;41(19):e186.   # First authorship
 
5. Miyanari Y, Torres-Padilla ME.
Control of ground-state pluripotency by allelic regulation
of Nanog. Nature. 2012 Feb 12;483(7390):470-3
 
6. Miyanari Y, Torres-Padilla ME.
Epigenetic regulation of reprogramming factors towards
pluripotency in mouse preimplantation development.
Curr Opin Endocrinol Diabetes Obes. 2010 Dec;
17(6):500-6
 
7. Hiura H, Sugawara A, Ogawa H, John RM, Miyauchi N,
Miyanari Y, Horiike T, Li Y, Yaegashi N, Sasaki H, Kono T,
Arima T. A tripartite paternally methylated region within
the Gpr1-Zdbf2 imprinted domain on mouse

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